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The Q Exactive mass spectrometer (Thermo Fisher Scientific, San Jose, CA, USA) was operated independently in positive or negative ion mode, scanning in Full MS mode (2 scans) from 60 to 900 m/z at 70,000 resolution, with 4 kV spray voltage, 45 shealth gas, 15 auxiliary gas

Vitamin A (as Retinyl Palmitate), Vitamin D (as Cholecalciferol), and the Vitamin E Isomers (DeltaGold tocotrienols) require the presence of dietary fats to stimulate the release of bile acids, which emulsify the vitamins and allow them to be absorbed through the intestinal wall