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Ajani JA, Xu Y, Huo L, Wang R, Li Y, Wang Y, Pizzi MP, Scott A, Harada K, Ma L, Yao X, Jin J, Zhao W, Dong X, Badgwell BD, Shanbhag N, Tatlonghari G, Estrella JS, Roy-Chowdhuri S, Kobayashi M, Vykoukal JV, Hanash SM, Calin GA, Peng G, Lee JS, Johnson RL, Wang Z, Wang L , Song S

Originally developed as a cancer drug, methotrexate stops cancerous cells from rapidly multiplying and spreading by blocking their access to folate, a form of vitamin B

[email protected] These authors share first authorship This article was submitted to Nutrition and Metabolism, a section of the journal Frontiers in Nutrition Disclaimer All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers

RIA radioimmunoassay ELISA enzyme-linked immunoabsorbent assay binding affinity of a monoclonal antibody or portion thereof also can be determined by the Scatchard analysis of Munson et al