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The cDNA fragments went through the ligation process with unique molecular identifier adapters synthesized by IDT

Govindarajan, R., Berry, J

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For HPLC-MS analysis a ZIC-pHILIC column (SeQuant, VWR) was used and Exactive and Q-Exactive mass spectrometers (Thermo Fisher Scientific) were operated with electrospray (ESI) ionisation and polarity switching mode at scan range ( m/z ) 751000 at a resolution of 25,000 at 200 m/z