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doi: 10.1016/j.actbio.2022.03.001

10.1038/sj.bjc.6690419 49 GuanB.HoqueA.XuX

Strains were grown in minimal SCD media (2% glucose, 0.67% YNB without amino acids (Difco)

However, as cysteine serves as a rate-limiting precursor for both glutathione biosynthesis and disulfide bond formation in the endoplasmic reticulum (ER), this redirection of cysteine, despite minimal changes in extracellular cysteine levels, can reduce the pool available for efficient IgG folding 41