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The mixture was then incubated for 10 min at 4 o C (obtained by nesting the mixture in wet ice)

Zeng K, Tian L, Patel R, Shao W, Song Z, Liu L, Manuel J, Ma X, McGilvray I, Cummins C, Weng J, Jin T (2017) Diet polyphenol curcumin stimulates hepatic Fgf21 production and restores its sensitivity in high-fat-diet-fed male mice

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[36] Vitamin B 12 deficiency is most commonly caused by malabsorption, but can also result from low intake, immune gastritis, low presence of binding proteins, or use of certain medications