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RNA quality and concentration assessment were performed using a Nanodrop 2000 spectrophotometer (Thermo Fisher Scientific) and Agilent 2100 Bioanalyzer with a 2100 RNA nano 6000 assay kit (Agilent Technologies, USA), respectively

TMA can be oxidized to TMAO through the liver and intestines (jejunum and cecum) and enter the blood circulation

The membranes were stained with 0.5% Ponceau S (Beyotime Institute of Biotechnology) to assure equal protein loading, blocked for 1 h with 5% powdered non-fat dry milk in 25 mM Tris-HCl (pH 8.0), 144 mM NaCl and 0.1% Tween 20 (TBS-T), and incubated overnight at 4C with the following primary antibodies: PPAR-, PPAR-, NF-B P65 (cat

Dont use drying products, like exfoliating acids