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National Institutes of Health Guide for the Care and Use of Laboratory Animals (NIH publication 86-23, revised 1996)

Nat Immunol (2008) 9(8):84756

After 4 h, the tobramycin-treated biofilms were washed twice with PBS, followed by either staining with crystal violet for biofilm biomass quantification or scrapping off adhered cells and incubating on TSB agar (as outlined above)