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During a follow-up period of more than 20 years, the RCS models demonstrated significant nonlinear associations of both serum folate ( P high school degree), marital status (married, unmarried), family income to poverty ratio ( 5), smoking status (current smoker, ex-smoker, never smoker), physical activity (active, median, inactive), Healthy Eating Index, and self-reported general health (excellent, very good, good, fair, poor)

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ELISA kits (R&D Systems, Abingdon, UK) for CCL3 (#DY270) and IL-1B (#DY201) were used and assays performed as per the manufacturers instructions

Unlike DSIP, Z-drugs have well-established efficacy but carry risks of tolerance, dependence, cognitive impairment, and residual daytime sedation