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Transient Transfection of Human GSTP1 Gene After plating on collagen-coated plates for 24 h, HepG2 cells were transiently transfected with 0.1 g/1 10 4 cells hGSTP1 expression plasmid (SC119655, Origene, Rockville, MD, United States) or accompanying empty pCMV6-XL5 vector (pCMV6-XL5) using the GenJet In Vitro Transfection Reagent for HepG2 cells (SignaGen, Rockville, MD, United States) according to the manufacturers instructions

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Correlating downregulated DEGs by RNA-seq and genes with decreased H3K4me3 ChIP-seq enrichment at the promoter showed an overlap of 51 genes, which are involved in several oncogenic pathways such as RAS and FGFR4 signalling (Fig

N-terminally truncated isoforms (N) lack the transactivation domain and appear to act in a dominant-negative manner by either competing with the TA isoforms of p63 and p53 for DNA binding sites or by direct protein interaction