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In homeostasis conditions, GSH is the most efficient physiological reducing agent with the highest bioavailability (~ 10 mM) in the intracellular environment where it is synthesized, except in epithelial cells [23]

The elution followed a linear gradient at a flow rate of 0.3 mL/min under the following conditions: 520% B from 0 to 1 min, 2025% B from 1 to 5.5 min, 2530% B from 5.5 to 6 min, 30% B from 6 to 8 min, 3035% B from 8 to 9 min, 3565% B from 9 to 17 min, 65100% B from 17 to 18 min, 100% B from 18 to 19 min, and 5% B from 19.1 to 20 min

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They found that the treatment reduced electrolyte leakage and positively affected the concentration of organic solutes