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Shaking creates shearing forces and foam, which can denature the peptide chains, essentially ripping the complex folded structure apart

CLINICAL MANIFESTATIONS OF THALASSEMIA SYNDROMES Mutations causing thalassemia can affect any step in the pathway of globin gene expression: transcription, processing of the mRNA precursor, translation, and posttranslational metabolism of the -globin polypeptide chain

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They had no concept of germ theory, but they could observe that the Chinese, living in far more crowded settlements than the northern nomads, often got the milder form of smallpox when young