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It has been the subject of over 130 peer-reviewed publications since the 1990s

For WB, 30 g of proteins from tissue and cell lysates were mixed with 8 L of sample buffer (Tris 0.5% (pH 6.8), glycerol 20%, sodium dodecylsulfate (SDS) 10%, 2--mercaptoethanol, bromophenol blue 0.1%), and RIPA buffer to a total sample volume of 32 L

Fred Schaufele, XCellAssay Research Summary: This project aimed at developing a cell-based nuclear receptor translocation bioassay has largely achieved the projected goals

Samples containing 20 g of protein were run on an SDS-PAGE gel and transferred to polyvinyl difluoride (PVDF) membranes (Immobilon-F, Millipore) using a transfer tank (TE22, Hoefer)