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Theor Appl Genet

100 L of 50% MeOH(aq) spiked with qualitative internal standards (qIS), 0.125 g/mL nitrodracylic acid and 0.125 g/mL isocaramidine sulfate was added to the extract solution, and then vortexed and incubated on ice for 10 min

FIGURE 2 3.3 Hypoxic-glucose deprivation conditions can induce senescence in endothelial cells Unlike the previously mentioned natural aging models and progeria models, there is another common but often overlooked endothelial cell aging model: hypoxia-induced endothelial cell senescence