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Major limitations include difficulties in maintaining the complex three-dimensional architecture of seminiferous tubules, establishing appropriate hormonal microenvironments, and sustaining long-term culture conditions necessary for the extended timeline of human spermatogenesis

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Fixed schedule regimens may be supplemented with additional diazepam as needed for people with low tolerance of withdrawal discomfort (for example, 5 mg 6 hourly as needed, based on clinical observation or alcohol withdrawal scale scores)