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J Sexual Med (2022) 19(2):2016

It modulates BDNF and other neurotrophic factors supporting neural health

130 In BMSCs and preosteoblast MC3T3-E1 cells, miR-376b-3p directly targets YAP1 to suppress YAP1 expression, and circ_0024097 serves as a ceRNA to rescue YAP1 by absorbing miR-376b-3p, leading to activation of the Wnt/-catenin signalling pathway and cell differentiation, which attenuates osteoporosis

2.8 Enzyme-linked immunosorbent assay (ELISA) Whole blood samples were centrifuged at 4C, 6,000 rpm for 10 min, and the supernatant was taken in a new tube next, and the supernatant was centrifuged again at 4C, 6,000 rpm for 10 min to remove impurities, and then plasma was collected