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When administered under medical supervision with appropriate screening and monitoring, NAD IV therapy is generally well tolerated for suitable candidates

Chromatographical separation of metabolites was performed on Syncronis HILIC UHPLC column (2.1 mm 100 mm 1.7 m, Thermo Fisher) at the flow rate of 600 L/min using a binary solvent system: solvent A, 20 mM ammonium formate, pH 3.0 in mass spectrometry grade H 2 0, and solvent B, mass spectrometry grade acetonitrile with 0.1% formic acid (%v/v)