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E2E1-I53-50A contained elevated levels of oligomannose at positions 430, 448, 476, and 623 possibly reflecting steric occlusion of these sites to mannosidase because of the trimeric organization of E2E1

Taken together, it is important to understand the working mechanisms and potentially beneficial effects of GHK-Cu complexes on protecting against cardiotoxicity, especially in zebrafish embryos, triggered by waterborne Cu exposure at different dosage concentrations and time periods, and even in various physiological environment conditions

degree from Mahidol University

Cell viability and proliferation assays Parental E0771, M-Wnt, and metM-Wnt lung cells, as well as GPX4- and PC-knockdown and shScramble control E0771 and metM-Wnt lung cells, were seeded at 5 10 3 cells/well in a 96-well plate in HPLM overnight, then treated with a ferroptosis inducer (erastin or RAS-selective lethal 3 (RSL3) [26, 27]) or carboplatin across a range of concentrations, antioxidants (1mM N-acetyl cysteine, 2.5 M liproxstatin-1 [28], 2.5-5 M ferrostatin-1 [26]), or a general ROS inducer H 2 O 2 (500 M)