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Cells were then incubated for 4 min at room temperature, centrifuged at 1000 x g for 3 min, washed once with 2 mL fresh HC -Ura-Leu medium and finally resuspended in fresh HC -Ura-Leu medium to an OD 600 of 7.5

Results are determined using a microplate reader

Neil Kaplowitz, professor of medicine and chief of the gastroenterology and liver diseases at the Keck School of Medicine of the University of Southern California, who was not involved in the study, said: The study authors developed a novel approach to dynamic assessment of the status of glutathione in the liver sinusoids

Grewe M, Tsiotos GG, Luque de-Leon E, et al