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Description

Following blocking with 2% bovine serum albumin in PBS, the cells were incubated with primary antibodies used at a dilution of 1:100 (ZO-1) overnight

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In fact, circulating adducts between hemoglobin and NAPQI have been detected, indicating some NAPQI even escapes hepatocytes, diffuses into RBCs, and combines with hemoglobin during its short existence