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Diagnosis of inborn errors of metabolism by acylcarnitine profiling in blood using tandem mass spectrometry

Finally, the proteomics approach has a few methodological limitations

The oxidative stress, typically observed in pathological conditions determining endothelial dysfunction, might induce endothelial NO depletion and reduced endothelial NO availability, mediated by the interaction of reactive oxygen species with NO to generate oxidative agents potentially damaging cell DNA and proteins, namely reactive nitrogen-containing species [51], or by a direct decrease of NO synthesis, therefore suggesting potential benefit from the administration of NOS substrates, such as l-ARG, as a therapeutic strategy to increase NO production and improve CVD-related endothelial dysfunction [19, 52]

Creatine kinase facilitates the reversible reaction between creatine and ATP, resulting in the formation of phosphocreatine and ADP, which are stored in muscle 49