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Free thiol assay The analyzed samples were incubated for 15 min at room temperature in 20 mM Tris-HCl buffer, pH 7.5, NaCl 100 mM, 1 mM EDTA, 5 % DMSO in the presence of 0.5 mM Ellmans Reagent (DTNB, 5,5-Dithio-bis-(2-nitrobenzoic acid), Sigma-Aldrich, Israel), and the absorbance was measured at 412 nm using the Infinite 200 PRO plate-reader (Tekan, Switzerland)

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Findings Here Whilst dogs can synthesise their own Vitamin C, there is no reason you cant add Vitamin C rich foods to the bowl to boost their levels