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Engineering novel ionizable lipids with tunable clearance properties for lipid nanoparticles: in their latest work, Sathyajith Kumarasinghe, Juneyoung Lee, Mohindra Seepersaud at Moderna and collaborators investigated the lipophilic properties of ester-linked squaramide ionizable lipids as predictors of in vivo lipid clearance from lipid nanoparticles

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Lactulose (0.5 mg/mL in HBSS, 100 L) was added to the apical side, and monolayers incubated for 4 h at 37C