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ChIP assays were performed using Kasumi-1 and K562 cells, as well as HEK293T cells transduced with GFP-ASXL1 1-645 -WT, GFP-ASXL1 1-645 -I574D, GFP-ASXL1 1-591 -WT or GFP-ASXL1 1-591 -I574D (5 10 cells per condition), following protocols 39

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