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Description

The potent antitumor effects of AC could be linked to its structural elements, particularly its phenolic hydroxyl groups (Vasincu et al

The proteins were precipitated again with 10% TCA, washed twice with acetone and the pellet resuspended in alkylation buffer (2% SDS, 100 mM Tris pH 8.0 containing 5 mM malPEG-2000 (NOF Corporation, Japan)) at 37C for 1 hour