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Inhibition of CPS1 or reduction of ammonia levels effectively suppresses collagen production and mitigates fibrosis in experimental models [80]

Cells or organoids were solubilized in pull-down buffer (50 mM Tris pH 7.3, 150 mM NaCl, 1% Triton X-100, 0.1 mM CaCl 2 plus protease/phosphatase inhibitors as above) and centrifuged at 14,000 rpm for 10 min at 4C

This target-centric research approach has provided valuable insights into the precise disruption of bacterial cell functions by antibiotics (Sodhi et al., 2023), an excessive reliance on this singular perspective also imposes certain limitations

Why the Glp pathway, and presumably MGO production, is upregulated in the presence of GO remains to be deciphered