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DNA extraction Total DNA was isolated from approximately 250350 mg of homogenized fecal samples using the QIAamp Fast DNA Stool Mini Kit (Qiagen, Hilden, Germany) as per manufacturers instructions, with the following modifications

Subsequently, we assessed the targeting ability of P-Mn/TI@mHSA toward atherosclerotic lesions following intravenous injections

SLC25A15 has been identified as a tumor suppressor in HCC, and its deficiency leads to reprogramming of glutamine metabolism, which promotes cancer cell development [16]

The partially purified and refolded scFv was further purified using Ni-NTA (Qiagen), followed by size-exclusion chromatography using a Superdex S200 increase 10/300 GL size-exclusion column (Cytiva) in HBS (10 mM HEPES, pH 7.2, 150 mM NaCl)