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10.1136/bmjresp-2021-000932 19

249 In patients with TNBC who have received neoadjuvant chemotherapy, researchers utilized scRNA-seq to identify that chemotherapy can induce the accumulation of ICOSL+ B cells

Then 1 10 9 cells were resuspended in 500 l yeast breaking buffer (50 mM sodium phosphate, 2% protease inhibitor cocktail (Sigma P8215), 1 mM EDTA, and 5% glycerol (v/v)) and an equal volume of acid-washed glass beads were added