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2.4.8 DNA extraction from homogenized tissue Total DNA was extracted from intestinal mucosa and lymph nodes as previously described ( 2.4.9 qPCR for identification of MAP DNA from tissue and fecal samples To detect MAP genomes in DNA samples, a two-duplex real-time qPCR assay targeting IS900 and F57 was employed ( 2.5 Ex-vivo trial to evaluate the functionality of effector immune cells in killing wild-type MAP 2.5.1 Preparation of antigen presenting cells to prime effector immune cells The ex vivo trial was designed and conducted as previously described ( Figure 1D )

Why the Other Choices Are Wrong Gout is an acute monoarticular arthritis caused by monosodium urate crystal deposition, often triggered by purine-rich foods like seafood and beer

261 National Research Council (2010)

Inflammatory cytokines such as IL-1 and TNF accelerate the production of the chemokines CXCL1, CXCL5, and CCL5 from fibroblast-like synovial cells, endothelial cells, and macrophages