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Neutral red staining was used to evaluate macrophage response, and RT-PCR was conducted to quantify key inflammatory cytokines (TNF-, IL-1, IL-10) and antioxidant gene expressions

Larger cerebral vessels are surrounded by mural cells, including vascular smooth muscle cells (VSMCs) around the larger vessels, which transition to pericytes while reaching the capillary bed, with a notable high ratio of pericytes to endothelial cells [10, 18]

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