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Although numerous small molecules evaluated as antioxidants have exhibited therapeutic potential in preclinical studies, clinical trial results have been disappointing

14.1 Consensus statements 14.1.1 Approximately 721% of children with autoimmune hepatitis presents as acute liver failure (LOE 4)

The perfusion solution was then switched to solution B (composed of 67 mM NaCl, 6.7 mM KCl, 10 mM HEPES, 0.5% BSA, 4.8 mM CaCl 2 2H 2 O (all provided by Sigma-Aldrich), and 1 g l 1 collagenase P (Roche) in ddH 2 O (pH 7.6), diluted 1:2 in stop solution (composed of DPBS with Ca 2+ , Mg 2+ (Gibco), supplemented with 16.7% FBS (Merck)) and perfused at 39 C for 515 min at a rate of 25 ml min 1