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EFSA NDA Panel (EFSA Panel on Dietetic Products, Nutrition and Allergies), 2015

We found that intracellular 1-MNA and NNMT protein expression levels were substantially higher in the fully differentiated adipocytes compared to the undifferentiated pre-adipocytes, which is consistent with the differentiation-dependent enhancement in NNMT mRNA levels and enzymatic activity reported previously in the 3T3-L1 cell lines.[37] The higher levels of 1-MNA and NNMT in adipocytes compared to pre-adipocytes may partially account for our observations that increasing concentrations (10 60 M) of NNMT inhibitor did not completely eliminate 1-MNA in adipocytes, but did fully suppress 1-MNA accumulation in undifferentiated pre-adipocytes

any ovary with a significantly greater weight (20% above the average weight of all enrolled ovaries) will be excluded

Non-rodent animal models for IVD include rabbits, which display a similar disc morphology to humans and have been used to study degeneration as well as potential therapeutics (Kroeber et al., 2002