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The membranes were then stained with Ponceau S and imaged to serve as a loading control for total protein normalization of Western blots

To clarify the causal relationships and stagedependent roles of these factors, we propose a conceptual framework for AD progression (1):Early AD (causal events): Disruption of cerebral iron homeostasis leads to regional iron accumulation, which primes neurons for ferroptosis through elevated ROS and impaired antioxidant defenses (2).Transitional AD (facilitative events): Ferroptotic stress promotes A aggregation and tau hyperphosphorylation

The assay mixture contained 2 mL of solution (800 mol) and 2.5 ml of phosphate buffer in a 10 mL flat bottom flask

Biosci Biotechnol Res Asia 20(4):14871499