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ROS measurements AuxB1 and CHO R C5 cells were seeded at a density of 50,000 cells/well in a clear-bottomed black-well plate and allowed to adhere for 24 h, after which the media was removed, and cells incubated with 100 M H 2 DCFDA for 45 min at 37 C

The reaction between RT and GSH is based on a Michael addition reaction that is inherently reversible, has an appropriate dissociation equilibrium constant K d in the mM range and rapid reaction kinetics, and provides ratiometric readouts, thus allowing GSH quantification independent of the probe concentration

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