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Bone marrow Microenvironment contributes to Stem Cell Phenotype in Acute Myeloid Leukemia

Freestone recommends cleaning reusable bottles with hot water (over 60C (140F) as this temperature kills most pathogens), and using washing-up liquid swirling it around, and leaving the bottle for 10 minutes before rinsing well with hot water

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The transfection of the pre-designed MISSION esiRNA (Merck) was performed with RNAiMAX (Invitrogen) according to the manufacturers instructions, with a final concentration of 30 pmol of esiRNA per well in 6-well plates