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Lysates were concentrated to a final concentration of 1 mg ml 1 by boiling the appropriate amount of protein lysate with 2 Laemmli buffer (4% SDS, 20% glycerol, 10% 2-mercaptoethanol and 0.004% bromophenol blue in 0.2 M Tris-HCl, pH 7) at 90 C for 10 min

This approach promises to deepen our understanding of laminopathies, bridging gene expression with protein functionality for a more comprehensive view of these conditions.We further discovered that LMNA (L35P) fish showed improvements in swim speed and muscle endurance following l-carnitine treatment

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