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In vitro epithelial infection model For our infections, the medium was removed and replaced with prewarmed fresh RPMI-1640 (or RPMI-1640 with L-glutamine but without glucose, catalog number 11879020, Thermo Fisher Scientific) without FBS, and supplemented with or without 10 mg/mL albumin

While scientists historically viewed cysteine as the primary limiting factor for this process, recent research on aging and oxidative stress reveals that endogenous glycine synthesis often falls drastically short of cellular demands, making glycine the true bottleneck for antioxidant production

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This patient's profound hand weakness, intrinsic muscle atrophy, and higher sensory loss make a T5 lesion unlikely