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Luria and Delbrck's finding that natural selection affects bacteria had major ramifications for molecular biology, helping, for example, to explain how bacteria become resistant to antibiotics (Greenspan, Kukan)

Participants were often pre-selected based on their ability to tolerate and engage with the technology, benefiting from close supervision and technical support

After 24 h incubation at 25 C, cells were harvested by 10 min centrifugation at 6000 g, 4 C

As the macrocycle is systematically varied from 24-crown-8 to benzo-24-crown-8 and further to dibenzo-24-crown-8, higher temperatures are required to achieve comparable half-lives