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The GC and MFC cells that underwent transfection were subjected to puromycin screening at a concentration of 2 g/ml for 24 h to establish stable GC and MFC cell lines capable of expressing ILF3

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Because this process does not require enzymatic catalysis, it is described as non-enzymatic lysine lactylation. This modification has been identified in hepatocellular carcinoma, where it promotes tumor proliferation and metastasis (Gaffney et al., 2020