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After centrifuging the mixture for 10 min at 6000 rpm and 4 C, the supernatants were adjusted to a known volume and used for catalase (CAT) assay

For NAC intervention experiments, rats were co-treated with N-acetylcysteine (NAC, Sigma Aldrich

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This stability ensures that the product can be effectively utilized for extended periods, allowing researchers to conduct experiments or analyses without time constraints or concerns about the degradation of the complex