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Abbreviations % DL : Drug loading % EE : Encapsulation efficiency 8-OHdG : 8-Hydroxy-2-deoxyguanosine ANOVA : Analysis of variance ApoE : Apolipoprotein E ApoE-MiADMSA-LPS : ApoE-coated monoisoamyl dimercaptosuccinic acid-loaded liposomes BBB : Blood-brain barrier BBD : Box-Behnken design CAT : Catalase CCSEA : Committee for Control and Supervision of Experiments on Animals COX : Cyclooxygenase Cp : Ceruloplasmin Cu : Copper CuSO 4 : Copper sulfate CV : Central vein DAPI : 4,6-Diamidino-2-phenylindole DMEM : Dulbeccos modified Eagle medium DMSA : Dimercaptosuccinic acid DMSO : Dimethylsulfoxide DSC : Differential scanning calorimetry EDTA : Ethylenediaminetetraacetic acid ELISA : Enzyme-linked immunosorbent assay EPM : Elevated plus maze FBS : Fetal bovine serum FITC : Fluorescein isothiocyanate FTIR : Fourier transform infrared spectroscopy GSH : Glutathione H&E : Hematoxylin and eosin stain IAEC : Institutional animal ethics committee LPS : Liposomes MiADMSA : Monoisoamyl dimercaptosuccinic acid MDA : Malondialdehyde MiADMSA-LPS : Monoisoamyl dimercaptosuccinic acid-loaded liposomes MTT : 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide MW : Molecular weight MWM : Morris water maze NORT : Novel object recognition test PBS : Phosphate buffer saline PDI : Polydispersibility index RNS : Reactive nitrogen species ROS : Reactive oxygen species SD : Standard deviation SEM : Scanning electron microscopy SOD : Superoxide dismutase TEM : Transmission electron microscopy TETA : Triethylenetetramine WD : Wilson disease References Azmand MJ, Rajaei Z (2021) Effects of crocin on spatial or aversive learning and memory impairments induced by lipopolysaccharide in rats

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Step 3: Load the Syringe Puncture the vial stopper and draw up the prescribed dose