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The experimental program that is used forHif 1 , caspase 3 and PDHB: Through with methanol incubation 30 minutes, then with 2 times (each 10 minutes) of TBS-T washing, use the ProBloot membrane strip buffer subsequently in 50 of following incubations 30 minutes, peel off the actin trace with 100ml or more TBS-T washing 2 times (each 30 minutes) then.The scanning trace is to check out fully stripped in laser scanner.With methanol activation trace 5 seconds, with water washing 5 minutes, then with TBST washing 15 minutes.At room temperature with the closed reagent among 5% TBS-T sealing trace 1 hour, subsequently with TBS-T washing 3 times (1 time, about 15 minutes

The composition of claim 4, wherein the polynucleotide encodes at least one cleavage site and the at least one cleavage site is a proteolytic cleavage site

Neesencijalne pak tijelo moe samostalno stvarati, no ravnotea izmeu obje skupine je izuzetno vana za optimalno funkcioniranje organizma