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After three PBS washes, iHLOs were incubated in 100 L Ce3D permeabilization/blocking buffer (BioLegend, catalog: 427706) for 1 h at room temperature

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Categorical variables were analyzed by either Fisher's test or Pearson's chi-squared test

Although viral vectors are the most effective approach for gene delivery, their use is limited due to their low safety, limited DNA loading capacity, complex assembly, and high cost