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Stop the reaction by adding 0.6 ml of the 200 mM EDTA stock solution

4.5 Copper Copper (Cu) plays two main roles: it promotes melanogenesis and provides physiological protection against oxidative stress by participating in the destruction of free radicals through the formation of superoxide dismutase, which catalyses the dismutation of reactive oxygen species to oxygen (O 2 ) or H 2 O 2

Further to its role in detoxification, GSTP1 has a myriad of other diverse and important cellular functions [610]

At the same time, smoking induces systemic and local oxidative stress, impairing mitochondrial integrity and disrupting estrogen synthesis by downregulating aromatase and steroidogenic transport proteins