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To calculate the cell viability, the cultured cells were uniformly distributed (1 10 3 cells/well) in a cell culture plate and incubated for the next 24 h, and subsequently treated with different concentrations (101000 g/mL) of test and control compounds for the next 5 days similar to cell culture conditions

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Finally, network pharmacological analysis indicated that riboflavin may regulate bone metabolism through pathways like HIF-1 signaling pathway, p53 signaling pathway, AGE-RAGE signaling pathway, longevity regulating pathway, etc

The potential of simultaneously targeting c-MET and PD-1/PD-L1 to enhance or hinder the efficacy of ICIs in tumor immunotherapy remains an active area of investigation