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Separation was carried out using a non-isocratic method (40%-50% methanol over 10 min) at ambient temperature and a flow rate of 0.3 mL/min

segment 27-1147) 14 (ii) reconstructing the flexible loops not present in the starting structure and (iii) model glycan chains attached on the N-linked glycosylation sites in order to be able to (iv) compute the solvent-accessible surface area (SASA) and antibody-accessible surface area (AASA) and (v) predict the B-cell epitopes and glycan masking (Fig

On day 3, fresh medium containing the cocktail was added to the cell cultures, replacing 1.4 ml of the old medium

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