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Description

Errors here cascade through your entire protocol, causing incorrect concentrations, degraded peptides, and unreliable results

After the treatments, 20 OD 600 units of cells were harvested and fluorescence measurements were performed as described above

For each recording (15 laser stimulations), the cerebral blood flow (CBV) was normalized into a relative steady-state value (rCBV) and calculated as the following: rCBV = (CBV(t) - CBV 0 )/CBV 0 , where CBV(t) is the power doppler value t seconds after the start of laser sequence, and CBV 0 is the baseline in the ROI