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The resulting peptide mixtures were desalted using C18 spin columns, vacuum-dried, and reconstituted in 0.1% formic acid

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To test if perturbation of Pol I triggers mitochondrial stress responses, we knocked down rpoa-2 in worm strains lacking key regulators of either the mitochondrial unfolded protein response (UPR mt ) ( atfs-1 ) 44 or mitophagy (pink-1 ) 45 , but observed the same relative lifespan extension by rpoa-2 RNAi in wild-type and mutant animals (Fig