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doi: 10.1007/PL00010322

Kalluri R, Weinberg RA (2009) The basics of epithelial-mesenchymal transition

During the validation and optimization method, the internal standards dissolved in the four solvents were tested in a mixture of 100 % methanol, methanol/water (50:50) (v/v), methanol + 0.01 % acetic acid, and ammonium buffer pH 3.5 [H 2 O/ACN (90:10) (v/v) + 1 mM NH 4 (CH 3 COO)]