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Cells were harvested by centrifugation, resuspended in a 30 mM Tris-HCl pH 8.0, 1 mM EDTA, and 200 mM NaCl lysis buffer, and stored at 20C

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The reduction of other prototypical examples of redox signaling-mediated antioxidative enzymes is also involved in the pathomechanism of psoriasis, such as GSH, Px, CAT, and SOD [56, 57]

Physiol Behav 100:525534 Wakahara S, Konoshita T, Mizuno S, Motomura M, Aoyama C, Makino Y, Kato N, Koni I, Miyamori I (2007) Synergistic expression of angiotensin-converting enzyme (ace) and ace2 in human renal tissue and confounding effects of hypertension on the ace to ace2 ratio