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The importance of nonaxonal pressure targets was accentuated in a landmark study by Morgans group, which demonstrated that pressure-induced dendritic retraction and RGC loss occur in the presence of intact axons (62)

To confirm the MTT results, a replicate plate run in parallel was developed by adding 5 l of 2.5 M Calcein-AM (ThermoFisher Scientific) to selected wells and incubated 15 min at room temperature

UCP2 inhibition in ALS motor neurons alters bioenergetics but does not impact the redox status Previous investigations linked the uncoupling protein 2 (UCP2) to mitochondrial dysfunction in ALS 3,31,32 , so we tested the role of this protein in the bioenergetic alterations of ALS mice motor neurons observed in our study

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